Characteristic and expression of Hsp70 and Hsp90 genes from Tyrophagus putrescentiae and their response to thermal stress

Abstract The development of insects is critically affected by temperature, which therefore plays an important role in the control stored grain pests. Extreme temperature stress conditions lead to biological responses mites, such as synthesis heat shock proteins. Tyrophagus putrescentiae ( Tp ) a pest mite that has negative effects on both economy and health. Since T. population dynamics are strongly influenced present study we have cloned cDNA HSP70 HSP90 (referred TpHSP70-1 , TpHSP70-2 TpHSP90 determined their expression fluorescence real time quantitative PCR. TpHSP70 showed high homology with similar genes other species open reading frames TpHSP70-1, encoded proteins 665, 661 718 amino acid residues, respectively. Under thermal stress, TpHsp70-1 TpHsp90 was up-regulated at higher temperatures, suggesting defense against stress.